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Biotech uses fermentation to produce milk proteins without cows

Technology
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  • My wife has an anaphylactic allergy to whey. I guess we'll now be waiting for the day she has a reaction to "vegan" milk.

    😬 now that's a rough time. Perfect Day does disclaim milk allergenic on their website but I have no idea if they require their clients to include that on their consumer product packaging.

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    This would be cool now I just need some good no animal version of a fat cap and gristle

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    Next stop: synthetic cheese!

  • Surprising (and frustrating) that they didn't use a properly deodorized grade of oil. Do you mind sharing which products you tried? I'm curious

    I don't recall the first one (I tried it sometime late last year) but the more recent one was from Tomorrow farms. Now that I think about it, I suppose there's a possibility they both were from the same company with different branding.

    The good news is that the bitter sunflower flavor can be drowned out pretty handily using chocolate syrup (and as I mentioned it's texture is spot on), so despite my dissatisfaction with the milk on it's own, it makes a great chocolate milk.

  • 😬 now that's a rough time. Perfect Day does disclaim milk allergenic on their website but I have no idea if they require their clients to include that on their consumer product packaging.

    If it takes off it might still make it harder to find a decent dairy alternative for people with this kind of allergy.

  • If it takes off it might still make it harder to find a decent dairy alternative for people with this kind of allergy.

    this is why at the end of the day I hope we invent Star Trek replicators

    and allergy cures

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    I remember hearing about this during a lecture at uni, almost 10 years ago. I hope it will actually be available at some point

  • I remember hearing about this during a lecture at uni, almost 10 years ago. I hope it will actually be available at some point

    But what for? There's all sorts of plant-based "milk" and "cheese". In case of milk drinks, with superior qualities like shelf life.

    Edit: alright, some melty stringy cheesy cheese.

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    Fermentation-made milk substitute was available at supermarkets in Singapore (under the brand name Very Dairy, though the original product was from the startup Perfect Day). I really liked it---a lot nicer than oat milk for drinking straight up. Unfortunately it went off the shelves after a while, seems like demand wasn't high 😞

  • Next stop: synthetic cheese!

  • The foodtech will use yeast as hosts to initially produce casein and whey protein, two of the main proteins found in milk.

    Bioreacted casein would be a HUGE leap for cow-free dairy. I remember when Perfect Day started up they were having too much trouble producing casein, so their products couldn't be used to make stringy or melty cheese.

    As a former cheese addict who developed severe lactose intolerance, I'll just be waiting here for the synthetic cheese. (Please. I miss pain-free cheese so much 😭)

    Why not eat lactose free cheese?

  • But what for? There's all sorts of plant-based "milk" and "cheese". In case of milk drinks, with superior qualities like shelf life.

    Edit: alright, some melty stringy cheesy cheese.

    Plant based cheese is god awful, its congealed oil textured. Milks have been great though

  • Why not eat lactose free cheese?

    Certain cheese (mainly hard cheese) is theoretically zero lactose due to fermentation using it up, but I find that even most hard cheeses give me a reaction.

    I have a history of psychosomatic reactions to food, though. For example I couldn't eat beef, chicken, or pork for years after several food poisoning incidents in quick succession. Even though I knew the reaction was all in my head, the physical effects still happened. Super frustrating.

    However I have had 2 incidents where I had GI symptoms occur after eating something containing dairy where I didn't know until I looked at the ingredients list, so I know it's not 100% in my head.

    To my fellow LI sufferers I encourage you to experiment with hard cheeses, as there's plenty of people who have documented their success with those! 🙂

    (Just do it on a day where you have a bathroom always available, lol.)

  • Plant based cheese is god awful, its congealed oil textured. Milks have been great though

    Some are sure, but there are many different ways to make vegan cheese that produce wildly different results. IMO the cashew based ones are best. If you can find it at your local store, Myokos mozzarella for pizzas is absolutely divine.

  • None of those are synthetic cheese. Just imitation.

  • Certain cheese (mainly hard cheese) is theoretically zero lactose due to fermentation using it up, but I find that even most hard cheeses give me a reaction.

    I have a history of psychosomatic reactions to food, though. For example I couldn't eat beef, chicken, or pork for years after several food poisoning incidents in quick succession. Even though I knew the reaction was all in my head, the physical effects still happened. Super frustrating.

    However I have had 2 incidents where I had GI symptoms occur after eating something containing dairy where I didn't know until I looked at the ingredients list, so I know it's not 100% in my head.

    To my fellow LI sufferers I encourage you to experiment with hard cheeses, as there's plenty of people who have documented their success with those! 🙂

    (Just do it on a day where you have a bathroom always available, lol.)

    I'm not talking about hard cheese but cheese that specifically says "lactose free" in the packaging

  • Plant based cheese is god awful, its congealed oil textured. Milks have been great though

    My wife is a pretty talented vegan baker and cook. Some things I can’t even tell. I’m not vegan but one of our adult sons is so I’m able to taste the difference when she makes something for him. She tried to make a vegan cheesecake once. It was the most horrible thing I’ve ever put in my mouth. She thought so too so I’m not being cruel. The taste was ok I guess but the texture was a complete disaster. He seemed to like it though. And the vegan cheese we buy to put on things like lasagna, is passable at best. Most of it tastes like yellow soft plastic.

    So yeah I agree. But strangely enough we found this plant based heavy whipping cream from country crock that she makes stabilized whip cream from (think instant pudding and heavy whipping cream) and I think I actually prefer that to real heavy cream. It’s amazing.

  • I'm not talking about hard cheese but cheese that specifically says "lactose free" in the packaging

    Never actually seen any besides the cream cheese at Trader Joe's. Going to keep an eye out now

  • But what for? There's all sorts of plant-based "milk" and "cheese". In case of milk drinks, with superior qualities like shelf life.

    Edit: alright, some melty stringy cheesy cheese.

    I’ve tried really hard, and sampled many brands, but other than soy in lattes sometimes, I can’t stand plant based milk in my coffee. Some of it is fine stuff for other uses, or even just drinking, but it just doesn’t come close to the flavor I like as part of my wake up routine. And if my coffee isn’t good it sours my whole day, just a bit.

    I’d love to have something that tastes like milk and feels like milk and does the job of milk, but is vegan.

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    Copy of the article because I don’t respect Microsoft:

    With finite natural resources and a growing demand for food, the world must find ways to overcome this challenge. One proposal comes from a Brazilian startup that will produce milk proteins without the need for cows.

    Founded in 2023, Future Cow wants to transform the dairy market by using precision fermentation, a process that combines high technology, sustainability, and production efficiency.

    "Our mission is to make milk without a cow," summarizes Leonardo Vieira, the company's co-founder and CEO. "Precision fermentation is a technology similar to that used in the production of beer or wine."

    The entrepreneur explains that the technology involves identifying the genetic sequence in the animal's DNA that provides instructions for producing the milk protein. The sequence is then copied and encoded in a host, which can be a fungus, yeast, or bacterium. The host then multiplies in a fermentation tank with a calorie source for nutrition.

    The result is a liquid that—after being filtered and dried—is transformed into the initially programmed milk proteins.

    "These proteins serve as ingredients for the food and dairy industry, which can recombine the product to create various derivatives," he explains.

    The foodtech will use yeast as hosts to initially produce casein and whey protein, two of the main proteins found in milk. Casein is widely used in cheese and yogurt production, while whey is rich in protein and highly valued in the food supplement market.

    There are also other proteins in milk, each with specific applications.

    "One of them is lactoferrin, which is extremely difficult to produce using traditional methods," says Vieira. "It takes 10,000 liters of milk to obtain just one kilo of this ingredient."

    #From the laboratory to the market

    Future Cow began operating in the Supera Technology Park in Ribeirão Preto, São Paulo state. There, it produced the first grams of milk protein using precision fermentation. It was then selected to take part in the DeepTech Acceleration Program (PACE) of the Brazilian Center for Research in Energy and Materials (CNPEM) in Campinas.

    It is now testing the scaling of the process, which is a critical stage for biotech companies.

    "Ninety-five percent of biotechs fail when they leave a bench environment and go to a pilot plant or other relevant environment," recalls Vieira. "We're very optimistic that with the support of the CNPEM and the available infrastructure, we'll achieve the scale-up we need for the next stage."

    The startup does not intend to replace animal milk entirely; rather, it wants to create complementary solutions for the industry.

    "When precision fermentation began, it was all very black or white: the product was either animal or it wasn't animal. Now, we see more hybrid models," Vieira observes.

    According to Vieira, executives from large dairies claim to already purchase all the available milk on the market. "They can't increase production by 20% or 30% with just the traditional raw material," he says. "If they can mix our ingredient with the animal product to create a hybrid product and increase the scale, it'll be a significant gain," he says.

    Another relevant aspect is the decarbonization agenda of large companies.

    "Even if precision fermentation doesn't fully replace animal milk, a 10% or 20% reduction in the carbon footprint of large corporations in the food sector would already represent a considerable environmental impact," Vieira continues.

    #Brazilian potential

    The sector for alternative proteins produced by precision fermentation is still in its early stages, but startups specializing in the segment are already emerging around the world.

    "Each one follows a different technological route. There's variation in the type of host [fungus, yeast, or bacteria] and in the target proteins," says Vieira.

    The entrepreneur points out that Brazil is in a privileged strategic position to lead the global market.

    "Brazil is the only country in the world that has an abundance of water, sugar, and renewable energy, which are the three essential inputs for fermentation. It's a unique opportunity for the country," he points out. "With these characteristics, Brazil can take the lead in a strategic industry for the future of global food."

    Future Cow's technical and economic analyses show that producing milk proteins on a 300,000-liter scale will be less expensive than traditional production methods. He points out that when precision fermentation reaches an industrial scale with lower costs, it will disrupt the market: "If Brazil only focuses on traditional agriculture at that point, we'll be left behind."

    The researcher cites New Zealand as an example. The country has characteristics similar to Brazil's, and a significant portion of its gross domestic product (GDP) comes from milk exports.

    "They've already realized that the sector is going to change and are moving to avoid being left behind," he comments. "I've been trying to alert the Brazilian government authorities to this potential."

    #Future prospects

    Future Cow already has a functional strain and is now looking to increase production yields.

    "The more the strain produces, the more the unit price falls. So we're optimizing the fermentation processes," Vieira asserts. The expectation is that the product will be ready and available for sale by the end of 2026.

    Since the product is an ingredient, the company will not sell directly to the end consumer, but rather will act as a supplier to the food industry. This approach could facilitate the startup's entry into the market.

    Vieira explains, "As an ingredient, our product can be incorporated into existing products without facing a high entry barrier."

    The startup will initially market the proteins it has already developed before expanding to other varieties.

    "Only after the first commercialization will we develop other proteins," says the entrepreneur.

    The company is preparing to take part in VivaTech, an innovation fair that will be held in Paris, France, in June.

    "The technology already exists in other countries and at VivaTech we'll be able to show that Brazil has it too," says Vieira. "We can win over investors who realize that we can manufacture in Brazil and export to other locations. This kind of exposure abroad is uncommon for Brazilian companies."

    At the meeting, Future Cow aims to connect with the innovation ecosystem, raise awareness of the development of the technology in Brazil, and attract potential corporate partners. "We want to demonstrate that we're developing alternative proteins and, with this, attract multinationals from the dairy sector to be our clients."

    #Scientific entrepreneurship

    One aspect that Vieira highlights is the combination of skills at Future Cow. While he brings experience in business and entrepreneurship, his partner, Rosana Goldbeck, has a Ph.D. in food engineering from the State University of Campinas (UNICAMP) and has already studied meat cultivation in Brazil.

    He says, "This mix is an important differentiator, as it brings together someone who understands business and someone who understands the technology."

    According to Vieira, this is one of the main barriers preventing more innovations from Brazilian universities from becoming commercial products: "Brazil produces a lot of science, has many scientific articles, but most of them don't become businesses," he laments. "There need to be more connections between the academic environment and entrepreneurship in Brazil."

    Provided by FAPESP

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